A method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains

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dc.contributor.affiliationUniversidad Andres Bello
dc.contributor.affiliationUniversidad de Valparaiso
dc.contributor.affiliationUniversidad de Las Americas - Chile
dc.contributor.authorNavarro, Belen
dc.contributor.authorAlarcon, Mackarenna
dc.contributor.authorOsees, Maricarmen
dc.contributor.authorGomez-Alvear, Felipe
dc.contributor.authorSepulveda, Romina V
dc.contributor.authorHuerta, Jaime
dc.contributor.authorOpazo, Maria Cecilia
dc.contributor.authorAguayo, Daniel
dc.date.accessioned2022-05-30T15:48:57Z
dc.date.available2022-05-30T15:48:57Z
dc.date.issued2021
dc.description.abstractIsothermal Titration Calorimetry (ITC) is widely employed to assess antimicrobial affinity for lipopolysaccharide (LPS); nevertheless, experiments are usually limited to commercially available-LPS chemotypes. Herein we show a method that uses Differential Scanning Calorimetry (DSC) to characterize homogeneity artificial vesicles of LPS (LPS-V) extracted from isogenic mutant bacterial strains before analyzing the antimicrobial binding by ITC. This method allows us to characterize the differences in the Polymyxin-B binding and gel to crystalline liquid (beta alpha) phase profiles of LPS-V made of LPS extracted from Escherichia coli isogenic mutant strains for the LPS biosynthesis pathway, allowing us to obtain the comparable data required for new antimicrobial discovery. A method for:Obtaining LPS vesicles from isogenic mutant bacterial strains.Characterize artificial LPS vesicles homogeneity.Characterize antimicrobial binding to LPS. (C) 2021 The Authors. Published by Elsevier B.V.
dc.description.sponsorshipFondecyt [1171654]; Fondequip [EQM140174]; Fondecyt Postdoctorado [3200951]; Centro Interdisciplinario de Neurociencia de Neurociencia de Valparaiso [P09-022-F]; We are grateful to Fondecyt 1171654, Fondequip EQM140174, Fondecyt Postdoctorado 3200951 and The Centro Interdisciplinario de Neurociencia de Neurociencia de Valparaiso , P09-022-F .
dc.identifier.citationMethodsX, 8, 101474. https://doi.org/10.1016/j.mex.2021.101474
dc.identifier.doihttps://doi.org/10.1016/j.mex.2021.101474
dc.identifier.folio1171654
dc.identifier.folio3200951
dc.identifier.folioEQM140174
dc.identifier.issn2215-0161
dc.identifier.orcidhttps://orcid.org/0000-0003-4263-9294
dc.identifier.orcidhttps://orcid.org/0000-0001-6807-0820
dc.identifier.orcidhttps://orcid.org/0000-0001-8899-1440
dc.identifier.pmid34434873
dc.identifier.researcheridHOH-2917-2023
dc.identifier.researcheridKIJ-9949-2024
dc.identifier.researcheridITT-1749-2023
dc.identifier.rorhttps://ror.org/01qq57711
dc.identifier.rorhttps://ror.org/02zhyrb63
dc.identifier.rorhttps://ror.org/05j6ybs54
dc.identifier.rorhttps://ror.org/00h9jrb69
dc.identifier.rorhttps://ror.org/0166e9x11
dc.identifier.scopusauthorid57193732879
dc.identifier.scopusauthorid57193732568
dc.identifier.scopusauthorid57226486957
dc.identifier.scopusauthorid57218351479
dc.identifier.scopusauthorid55362357100
dc.identifier.scopusauthorid57193728352
dc.identifier.scopusauthorid55966517500
dc.identifier.scopusauthorid55211803600
dc.identifier.urihttps://repositorio.udla.cl/handle/udla/1108
dc.language.isoeng
dc.publisherElsevier BV
dc.relation.fundingFondo Nacional de Desarrollo Científico y Tecnológico, FONDECYT, (1171654, 3200951, EQM140174)
dc.relation.fundingFondo Nacional de Desarrollo Científico y Tecnológico, FONDECYT
dc.relation.fundingFondecyt [1171654]
dc.relation.fundingFondequip [EQM140174]
dc.relation.fundingFondecyt Postdoctorado [3200951]
dc.relation.fundingCentro Interdisciplinario de Neurociencia de Neurociencia de Valparaiso [P09-022-F]
dc.relation.isindexedbyWeb of Science
dc.relation.issn2215-0161
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.sourceMETHODSX
dc.source.urihttps://doi.org/10.1016/j.mex.2021.101474
dc.subjectDifferential Scanning Calorimetry (ITC)
dc.subjectIsothermal Titration Calorimetry (ITC)
dc.subjectAntimicrobial binding
dc.subjectLipopolysaccharides
dc.titleA method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains
dc.title.alternativeA method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains.
dc.typejournal article
dc.type.coarhttp://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.udla.catalogadorCBM
oaire.citation.titleMETHODSX
oaire.citation.volume8
oaire.fundingReference.awardNumber1171654
oaire.fundingReference.awardNumber3200951
oaire.fundingReference.awardNumberEQM140174
oaire.fundingReference.funderNameAgencia Nacional de Investigación y Desarrollo (ANID)
udla.curacion.controljmvg
udla.oecd.area1 Ciencias Naturales
udla.oecd.discipline1.6.2 Microbiología
udla.oecd.subarea1.6 Ciencias Biológicas

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