A method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains

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Navarro et al.2021.A method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains.pdf (950.65 KB)

Date

2021

Document type

journal article

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Authors

Navarro, Belen, Alarcon, Mackarenna, Osees, Maricarmen, Gomez-Alvear, Felipe, Sepulveda, Romina V, Huerta, Jaime, Opazo, Maria Cecilia, Aguayo, Daniel

Publisher

Elsevier BV

URI

https://repositorio.udla.cl/handle/udla/1108

DOI

https://doi.org/10.1016/j.mex.2021.101474

Keywords

Differential Scanning Calorimetry (ITC), Isothermal Titration Calorimetry (ITC), Antimicrobial binding, Lipopolysaccharides

Citation

MethodsX, 8, 101474. https://doi.org/10.1016/j.mex.2021.101474

Abstract

Isothermal Titration Calorimetry (ITC) is widely employed to assess antimicrobial affinity for lipopolysaccharide (LPS); nevertheless, experiments are usually limited to commercially available-LPS che...

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