The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes

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Date

2022-07-05

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journal article

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Investigación
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Authors

Luraschi, Roberto, Barrera-Avalos, Carlos, Vallejos-Vidal, Eva, Alarcon, Javiera, Mella-Torres, Andrea, Hernandez, Felipe, Inostroza-Molina, Ailen, Valdes, Daniel, Imarai, Monica, Acuña-Castillo, Claudio, Reyes-López, Felipe E., Sandino, Ana Maria

Publisher

WILEY

URI

https://repositorio.udla.cl/handle/udla/1460

DOI

https://doi.org/10.1155/2022/2594564

Keywords

Article, comparative study, controlled study, diagnostic test accuracy study, false negative result, gene amplification, human, limit of detection, nasopharyngeal swab, quantitative analysis, real time polymerase chain reaction, RNA extraction, Severe acute respiratory syndrome coronavirus 2, virus detection, virus gene

Citation

Canadian Journal of Infectious Diseases and Medical Microbiology, 2022, 2594564. https://doi.org/10.1155/2022/2594564

Abstract

The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed t...

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Creative Commons Attribution 4.0 International
Except where otherwised noted, this item's license is described as Creative Commons Attribution 4.0 International
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