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dc.contributor.authorAuthorLuraschi, Roberto
dc.contributor.authorAuthorBarrera-Avalos, Carlos.
dc.contributor.authorAuthorVallejos-Vidal, Eva.
dc.contributor.authorAuthorAlarcón, Javiera
dc.contributor.authorAuthorMella-Torres, Andrea.
dc.contributor.authorAuthorHernández, Felipe
dc.contributor.authorAuthorInostroza-Molina, Ailen.
dc.contributor.authorAuthorValdés, Daniel
dc.contributor.authorAuthorImarai, Mónica
dc.contributor.authorAuthorAcuña-Castillo, Claudio.
dc.contributor.authorAuthorReyes-López, Felipe E.
dc.contributor.authorAuthorSandino, Ana María
dc.date.accessionedDate Accessioned2024-09-03T19:19:22Z
dc.date.availableDate Available2024-09-03T19:19:22Z
dc.date.issuedDate Issued2022
dc.identifier.citationReferencia BibliográficaCanadian Journal of Infectious Diseases and Medical Microbiology, 2022, 11 p.
dc.identifier.issnISSN1712-9532
dc.identifier.uriURIhttp://repositorio.udla.cl/xmlui/handle/udla/1460
dc.identifier.uriURIhttps://onlinelibrary.wiley.com/toc/9575/2022
dc.description.abstractAbstractThe early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace the viral spread and the isolation of COVID-19-infected patients. The detection capacity of viral and internal genes is an essential parameter to consider and analyze during the assay. In this study, we analyze the performance of the two commercial RT-qPCR kits used in Chile, TaqMan™ 2019-nCoV Control Kit v1 (Thermo Fisher) and MaxCov19 (TAAG Genetics), for the COVID-19 diagnosis from nasopharyngeal swab samples (NPSs). Our results show a lower sensitivity of the TAAG kit compared to the Thermo Fisher kit, even in the detection of SARS-CoV-2 mutations associated with its variants. This study reinforces the relevance of evaluating the performance of RT-qPCR kits before being used massively since those with lower sensitivity can generate false negatives and produce outbreaks of local infections.
dc.format.extentdc.format.extent11 páginas
dc.format.extentdc.format.extent539.4Kb
dc.format.mimetypedc.format.mimetypePDF
dc.language.isoLanguage ISOeng
dc.publisherPublisherHindawi
dc.sourceSourcesCanadian Journal of Infectious Diseases and Medical Microbiology
dc.subject.lcshdc.subject.lcshCOVID-19 (Enfermedad)
dc.titleTitleThe Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes
dc.typeDocument TypeArtículo
dc.udla.catalogadordc.udla.catalogadorCBM
dc.udla.indexdc.udla.indexWoS
dc.udla.indexdc.udla.indexScience Citation Index Expanded
dc.udla.indexdc.udla.indexScopus
dc.udla.indexdc.udla.indexAcademic Search Ultimate
dc.udla.indexdc.udla.indexDOAJ
dc.udla.indexdc.udla.indexCAB Abstracts
dc.udla.indexdc.udla.indexEMBASE
dc.identifier.doidc.identifier.doi10.1155/2022/2594564
dc.facultaddc.facultadFacultad de Medicina Veterinaria y Agronomía


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