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Identification of exoenzymes secreted by entomopathogenic fungus beauveria pseudobassiana RGM 2184 and their effect on the degradation of cocoons and pupae of quarantine pest lobesia botrana
dc.contributor.author | Autor | Arias-Aravena, Matias | |
dc.contributor.author | Autor | Altimira, Fabiola | |
dc.contributor.author | Autor | Gutiérrez, Daniela | |
dc.contributor.author | Autor | Ling, Jian | |
dc.contributor.author | Autor | Tapia, Eduardo | |
dc.date.accessioned | Fecha ingreso | 2024-09-03T19:19:15Z | |
dc.date.available | Fecha disponible | 2024-09-03T19:19:15Z | |
dc.date.issued | Fecha publicación | 2022 | |
dc.identifier.citation | Referencia Bibliográfica | Journal of Fungi, 8(10), 16 p. | |
dc.identifier.issn | ISSN | 2309-608X | |
dc.identifier.uri | URL | http://repositorio.udla.cl/xmlui/handle/udla/1440 | |
dc.identifier.uri | URL | https://www.mdpi.com/journal/jof | |
dc.description.abstract | Resumen | Beauveria pseudobassiana RGM 2184 has shown 80% maximum efficacy against the pest Lobesia botrana in the autumn and winter seasons. This suggests that the strain possesses an interesting battery of enzymes that are cold-adapted to penetrate the thick and hydrophobic cocoon of L. botrana. In this study, screening of the proteolytic, lipolytic, and chitinolytic activity of enzyme extracts secreted by the RGM 2184 strain was carried out in various culture media. The enzyme extracts with the highest activity were subjected to zymography and mass spectrometry. These analyses allowed the identification of two proteases, two lipases, and three chitinases. Comparative analysis indicated that the degree of similarity between these enzymes was substantially reduced when the highest degree of taxonomic relatedness between RGM 2184 and the entomopathogenic fungus strain was at the family level. These results suggest that there is a wide variety of exoenzymes in entomopathogenic fungi species belonging to the order Hypocreales. On the other hand, exoenzyme extract exposure of cocoons and pupae of L. botrana provoked damage at 10 °C. Additionally, an analysis of the amino acid composition of the RGM 2184 exoenzyme grouped them close to the cold-adapted protein cluster. These results support the use of this strain to control pests in autumn and winter. Additionally, these antecedents can form a scaffold for the future characterization of these exoenzymes along with the optimization of the strain’s biocontrol ability by overexpressing them. | |
dc.format.extent | dc.format.extent | 16 páginas | |
dc.format.extent | dc.format.extent | 5.476Mb | |
dc.format.mimetype | dc.format.mimetype | ||
dc.language.iso | Lenguaje ISO | eng | |
dc.publisher | Editor | MDPI | |
dc.rights | Derechos | Creative Commons Attribution License (CC BY) | |
dc.source | Fuentes | Journal of Fungi | |
dc.subject | Palabras Claves | Beauveria pseudobassiana | |
dc.subject | Palabras Claves | Cocoon | |
dc.subject | Palabras Claves | Cold-adapted protein | |
dc.subject | Palabras Claves | Entomopathogenic fungi (EPF) | |
dc.subject | Palabras Claves | Exoenzymes | |
dc.subject | Palabras Claves | Lobesia botrana | |
dc.title | Título | Identification of exoenzymes secreted by entomopathogenic fungus beauveria pseudobassiana RGM 2184 and their effect on the degradation of cocoons and pupae of quarantine pest lobesia botrana | |
dc.type | Tipo de Documento | Artículo | |
dc.udla.catalogador | dc.udla.catalogador | CBM | |
dc.udla.index | dc.udla.index | WoS | |
dc.udla.index | dc.udla.index | Science Citation Index Expanded | |
dc.udla.index | dc.udla.index | Scopus | |
dc.udla.index | dc.udla.index | Academic Search Ultimate | |
dc.udla.index | dc.udla.index | Natural Science Collection | |
dc.udla.index | dc.udla.index | DOAJ | |
dc.udla.index | dc.udla.index | BIOSIS | |
dc.identifier.doi | dc.identifier.doi | 10.3390/jof8101083 | |
dc.facultad | dc.facultad | Facultad de Medicina Veterinaria y Agronomía |