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dc.contributor.authorAuthorArias-Aravena, Matias
dc.contributor.authorAuthorAltimira, Fabiola
dc.contributor.authorAuthorGutiérrez, Daniela
dc.contributor.authorAuthorLing, Jian
dc.contributor.authorAuthorTapia, Eduardo
dc.date.accessionedDate Accessioned2024-09-03T19:19:15Z
dc.date.availableDate Available2024-09-03T19:19:15Z
dc.date.issuedDate Issued2022
dc.identifier.citationReferencia BibliográficaJournal of Fungi, 8(10), 16 p.
dc.identifier.issnISSN2309-608X
dc.identifier.uriURIhttp://repositorio.udla.cl/xmlui/handle/udla/1440
dc.identifier.uriURIhttps://www.mdpi.com/journal/jof
dc.description.abstractAbstractBeauveria pseudobassiana RGM 2184 has shown 80% maximum efficacy against the pest Lobesia botrana in the autumn and winter seasons. This suggests that the strain possesses an interesting battery of enzymes that are cold-adapted to penetrate the thick and hydrophobic cocoon of L. botrana. In this study, screening of the proteolytic, lipolytic, and chitinolytic activity of enzyme extracts secreted by the RGM 2184 strain was carried out in various culture media. The enzyme extracts with the highest activity were subjected to zymography and mass spectrometry. These analyses allowed the identification of two proteases, two lipases, and three chitinases. Comparative analysis indicated that the degree of similarity between these enzymes was substantially reduced when the highest degree of taxonomic relatedness between RGM 2184 and the entomopathogenic fungus strain was at the family level. These results suggest that there is a wide variety of exoenzymes in entomopathogenic fungi species belonging to the order Hypocreales. On the other hand, exoenzyme extract exposure of cocoons and pupae of L. botrana provoked damage at 10 °C. Additionally, an analysis of the amino acid composition of the RGM 2184 exoenzyme grouped them close to the cold-adapted protein cluster. These results support the use of this strain to control pests in autumn and winter. Additionally, these antecedents can form a scaffold for the future characterization of these exoenzymes along with the optimization of the strain’s biocontrol ability by overexpressing them.
dc.format.extentdc.format.extent16 páginas
dc.format.extentdc.format.extent5.476Mb
dc.format.mimetypedc.format.mimetypePDF
dc.language.isoLanguage ISOeng
dc.publisherPublisherMDPI
dc.rightsRightsCreative Commons Attribution License (CC BY)
dc.sourceSourcesJournal of Fungi
dc.subjectSubjectBeauveria pseudobassiana
dc.subjectSubjectCocoon
dc.subjectSubjectCold-adapted protein
dc.subjectSubjectEntomopathogenic fungi (EPF)
dc.subjectSubjectExoenzymes
dc.subjectSubjectLobesia botrana
dc.titleTitleIdentification of exoenzymes secreted by entomopathogenic fungus beauveria pseudobassiana RGM 2184 and their effect on the degradation of cocoons and pupae of quarantine pest lobesia botrana
dc.typeDocument TypeArtículo
dc.udla.catalogadordc.udla.catalogadorCBM
dc.udla.indexdc.udla.indexWoS
dc.udla.indexdc.udla.indexScience Citation Index Expanded
dc.udla.indexdc.udla.indexScopus
dc.udla.indexdc.udla.indexAcademic Search Ultimate
dc.udla.indexdc.udla.indexNatural Science Collection
dc.udla.indexdc.udla.indexDOAJ
dc.udla.indexdc.udla.indexBIOSIS
dc.identifier.doidc.identifier.doi10.3390/jof8101083
dc.facultaddc.facultadFacultad de Medicina Veterinaria y Agronomía


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